Although aging is an inevitable part of the human process—recent findings suggest we may be able to slow down the biological processes associated with longevity. A new study recently released found that a theacrine-based supplement known as NAD3 was able to successfully alter sirtuin activity in vitro while affecting markers of mitochondrial biogenesis.
Hi, I’m Sean Torbati. As a health and well-being expert with a strong grasp of fundamental science—I believe that while aging can’t be stopped, individuals can take the proper steps to age more gracefully. In addition to proper diet and exercise—other steps can be taken to improve the biological processes associated with aging.
Let’s take a closer look at the study and the significance of these findings.
Results of the Study
A theacrine-based supplement known as NAD3 was examined and results showed it altered sirtuin activity in vitro while also affecting markers of mitochondrial biogenesis (1). A murine C2C12 myoblast cell line was used for experimentation and following 7 days of differentiation—myotubes were treated with 0.45mg/mL of NAD3 for 3 and 24 hours.
Compared to control groups NAD3 treatments increased Sirt1 mRNA levels at 3 hours and global sirtuin activity at 3 and 24 hours. Later experiments compared 24 h NAD3 or CTL treatments indicated NAD3 increased nicotinamide phosphoribosyltransferase (NAMPT) and SIRT1 protein levels. NAD+ levels were also elevated nearly two-fold after 24h of NAD3 versus CTL treatments.
The findings of the study suggest theacrine-based supplements may be able to modulate select biomarkers related to NAD+ biogenesis and sirtuin activity.
How Can Anti-Aging be Prevented?
NAD+ Boosting
NAD+, also known as nicotinamide adenine dinucleotide is an essential cofactor in all living cells that are involved in the fundamental biological processes (3). NAD+ depletion has been associated with hallmarks of aging and may be related to a wide-range of age-related diseases such as metabolic disorders, cancer, and neurodegenerative diseases. Evidence has even indicated that NAD+ may reverse aspects of aging and delay the progression of age-related diseases.
Sirtuin Boosting
Boosting sirtuin activity may help to extend lifespan. One of the substrates of the SIR2 ortholog, SIRT1, is a regulator of mitochondrial biogenesis (4). The SIRT1 activator resveratrol has been shown to help stimulate mitochondrial biogenesis while delivering health benefits in treated mice.
SIRT1-mediated mitochondrial biogenesis may help to reduce the production of reactive oxygen species, a possible cause of aging, and provide other mechanisms for the effect (5). Sirtuins play a critical role in regulating cellular homeostasis, keeping cells in balance while under the presence of NAD+, a coenzyme found in all living cells.
Sirtuins are considered a class of proteins that exist within various subcellular compartments and possess either mono-ADP-ribosyltransferase or deacetylase activity. Sirtuins have been found to play a critical role in the tissue aging process. Various research has indicated that NAD3 may be able to help activate sirtuins (2). This particular study showed that NAD3, as a theacrine-based supplement, affects molecular markers in skeletal muscle cells.
NLRP3 Activation
The NLRP3 inflammasome is involved in a broad spectrum of disorders including autoimmune diseases, type-2 diabetes, and neurological disorders such as Alzheimer’s disease and Parkinson’s disease (6). The NLRP3 inflammasome is linked to aged-related metabolic and neurodegenerative conditions.
NLRP3 is linked to several age-related diseases. Acting as a tumor suppressor in lung cancer—NLRP3 may provide therapeutic effects related to certain diseases such as human papillomavirus and cervical cancer (7).
How NAD3 Prevents Aging
Boosting NAD+ levels bring an array of health benefits related to better focus, memory, sleep, energy, and metabolism. HPN’s NAD3 helps to suppress the NLRP3 gene—a protein known to cause inflammation. Through suppression of NAD+, it can help to prevent the effects of aging.
- Activate sirtuins: the healthy-aging and survival protein
- Protect telomeres: increase telomerase activity to protect DNA
- NAD+ booster: boost NAD+ the crucial co-enzyme for mitochondrial function
- Cellular inflammation: influence NLRP3 expression to control cellular inflammation
At HPN we create products that work to improve physical and mental health and longevity. With a constant dedication and drive towards enhancing human lifespan and functionality—we remain committed to the health and welfare of our customers. If you have any additional questions or comments, please feel free to contact me for additional information or questions.
Kindest regards,
Sean Torbati
References:
- https://www.mdpi.com/2072-6643/12/12/3727/htm
- https://dx.doi.org/10.1016/j.metabol.2018.04.011
- https://www.sciencedirect.com/science/article/pii/S2468501118300063
- http://symposium.cshlp.org/content/72/483.short
- http://symposium.cshlp.org/content/72/483.short
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348816/
